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igg1  (SouthernBiotech)


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    SouthernBiotech igg1
    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 <t>(IgG1);</t> (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
    Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg1+fitc/Mouse+Anti-Human+IgG1+Hinge-FITC/pmc12934314-48-54-57
    Average 93 stars, based on 22 article reviews
    igg1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration"

    Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

    Journal: Journal of Translational Autoimmunity

    doi: 10.1016/j.jtauto.2026.100359

    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
    Figure Legend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Techniques Used: Immunofluorescence, Fluorescence, Control

    Related Articles

    other:

    Article Title: Cellular strain avoidance is mediated by a functional actin cap - observations in an Lmna -deficient cell model.
    Article Snippet: For double labelling of actin and myosin, and double labelling of myosin and phosphomyosin, goat anti mouse IgG1_FITC and goat anti mouse IgG2B-TxRd (ITK, Southern Biotechnology, USA) were used.

    Article Title: A preliminary study of the localization of infectious laryngotracheitis virus glycoprotein E within specific peripheral blood lymphocytes.
    Article Snippet: Infectious laryngotracheitis virus (ILTV) DNA has been detected in blood fractions, but the cell phenotype with which the virus is associated is unknown.. This study investigated the presence of ILTV antigen in peripheral blood cells of six acutely ILTV-infected chickens (5 or 9 days post ocular inoculation with a virulent isolate) and three sham-inoculated chickens using immunofluorescent staining.. Blood fractions were separated by Ficoll-Paque density gradient centrifugation, and smears were prepared from erythrocyte and leukocyte fractions.

    Article Title: Conserved functional features of natural killer cell subsets in chicken, human, and murine immune systems
    Article Snippet: Mouse IgG1 FITC , SouthernBiotech , Cat#0102-02; RRID: AB_2793846.

    Article Title: T cell subset profile and inflammatory cytokine properties in the gut-associated lymphoid tissues of chickens during infectious bursal disease virus (IBDV) infection.
    Article Snippet: While infectious bursal disease virus (IBDV) mainly targets immature B cells and causes T cell infiltration in the bursa of Fabricius (BF) of chickens, the effect of IBDV infection on the properties of T cells and relevant cytokine production in avian gut-associated lymphoid tissues (GALTs) remains unknown.. Here, we show that while the CD8+ T cell subset is not affected, IBDV infection decreases the percentage of CD4+ T cells in the cecal tonsil (CT), but not in esophagus tonsil, pylorus tonsil, and Meckel’s diverticulum of GALTs, in contrast to BF and spleen, in which the proportion of CD4+ cells increases upon IBDV infection.. Further, IBDV infection upregulates IFN-γ, IL-10, and the T cell checkpoint receptor LAG-3 mRNA expression in BF.

    Article Title: Comparative In Vitro and In Vivo Studies of Porcine Rotavirus G9P[13] and Human Rotavirus Wa G1P[8]
    Article Snippet: 250 Mouse IgG2a-PE and mouse IgG1-FITC (Southern Biotech, Birmingham, AL, 251 USA) were used as isotype controls.

    Staining:

    Article Title: Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells
    Article Snippet: After dissociation, cells were treated with ACK lysis buffer (A10492, Gibco, Grand Island, NY, USA) for 5 min to remove red blood cells. .. Cells (1 × 10 6 ) were stained for 40 min on ice using the following antibodies: mouse anti-chicken Bu-1 FITC (8395-02), mouse anti-chicken IgM BIOT (8310-08), mouse anti-chicken CD45 SPRD (8270-13), mouse anti-chicken CD3 FITC (8200-02), mouse anti-chicken CD4 Alexa Fluor 647 (8201-31), mouse anti-chicken CD8α BIOT (8405-08), mouse IgG1-FITC (0102-02), mouse IgM-SPRD (0101-13), mouse IgG2b-BIOT (0104-08) and mouse IgG1-Alexa Fluor 647 (0102-31) were purchased from Southern Biotech (Birmingham, AL, USA). .. After washed with PBS, BIOT cells were incubated with Bv421 conjugated secondary antibodies (BD sciences, 563259) for 20 min on ice.

    Article Title: Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells.
    Article Snippet: After dissociation, cells were treated with ACK lysis buffer (A10492, Gibco, Grand Island, NY, USA) for 5 min to remove red blood cells. .. Cells (1 × 106) were stained for 40 min on ice using the following antibodies: mouse anti-chicken Bu-1 FITC (8395-02), mouse anti-chicken IgM BIOT (8310-08), mouse anti-chicken CD45 SPRD (8270-13), mouse antichicken CD3 FITC (8200-02), mouse anti-chicken CD4 Alexa Fluor 647 (8201-31), mouse anti-chicken CD8a BIOT (8405- 08), mouse IgG1-FITC (0102-02), mouse IgM-SPRD (0101-13), mouse IgG2b-BIOT (0104-08) and mouse IgG1-Alexa Fluor 647 (0102-31) were purchased from Southern Biotech (Birmingham, AL, USA). .. After washed with PBS, BIOT cells were incubated with Bv421 conjugated secondary antibodies (BD sciences, 563259) for 20 min on ice.

    Incubation:

    Article Title: Flow Cytometric Evaluation of CD4 + and CD8 + T-cell Immune Response in SPF Chickens Induced by Fowlpox Vaccine
    Article Snippet: .. Briefly, 50 μL of the PBMCs (1 x 106 cells) was incubated for 30 min at 4 °C in a dark place with 10 μL of mouse anti-chicken CD3-Spectral Red (SPRD) and 10 μL mouse anti-chicken CD4-R-phycoerythrin (R-PE), or 10 μL mouse anti-chicken CD8a-R-PE (Southern Biotech, Birmingham, AL), and 10 μL mouse IgG1-FITC, respectively. .. All flow cytometry analyses were performed on a Partec PAS equipped with a 488 nm blue laser and a 640 nm red laser.



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    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 <t>(IgG1);</t> (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
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    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 <t>(IgG1);</t> (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).
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    Image Search Results


    Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Journal: Journal of Translational Autoimmunity

    Article Title: Anti-IgLON5 encephalitis is associated with anti-retinal immunological reactivity without retinal alteration

    doi: 10.1016/j.jtauto.2026.100359

    Figure Lengend Snippet: Indirect immunofluorescence on sections of monkey retina. The fluorescence observed is identified by a black arrow. (A) Patient 4 (IgG4); (B) Patient 1 (IgG4); (C) Patient 3 (IgG1); (D) Patient 5 (IgG4); (E) Patient 2 (IgG1); (F) Patient 1 (IgG4) after immunoadsorption of IgLON5 antibodies; (G) Control with macular edema (IgG1); (H) Control with anti-Hu encephalitis (IgG1); (I) Control with CAR syndrome (IgG4). The different layers of the retina are identified by their initials: pigment epithelium (pe), photoreceptor layer (pr), outer grain layer (og), outer plexiform layer (op), inner grain layer (ig), inner plexiform layer (ip), ganglion cell layer (gc), nerve fibre layer (nf).

    Article Snippet: The reactivity of the patients’ sera and/or CSF against the retina was evaluated retrospectively with frozen samples (−80°, EXPLAINEUR biobank) through an indirect immunofluorescence technique using sections of monkey retina (Ref. FA1172-1005, Euroimmun), detected with an FITC-labelled secondary antibody anti-human IgAGM (Euroimmun conjugate) or directed against IgA (ref. F0204, DAKO), IgM (ref. F0203, DAKO), IgG1 (ref. 9052-02, Southern Biotech) and IgG4 (ref. 9200-02, Southern Biotech).

    Techniques: Immunofluorescence, Fluorescence, Control

    a NRP2 expression in primary, THP-1 and Kasumi-3 monocytes and macrophages as measured by RNA-seq. Mono monocytes, Mac macrophages, RPM reads per million. n = 2. Source data are provided as a file. b Flow cytometry analysis of NRP2 versus IgG control cell surface staining in primary and THP1 monocytes and macrophages. c Flow cytometry analysis of THP1 macrophages with NRP2 or control CRISPR knockout, infected with HCMV-GFP. Analysis was performed at 3 dpi. d Flow cytometry analysis of THP1 macrophages, transfected with NRP2 and control siRNA 2 days before infection with HCMV-GFP. Analysis was performed at 3 dpi. Gating strategies for Fig. 6b–d are shown in Fig. .

    Journal: Nature Communications

    Article Title: Viral entry shapes HCMV latency establishment

    doi: 10.1038/s41467-025-68063-y

    Figure Lengend Snippet: a NRP2 expression in primary, THP-1 and Kasumi-3 monocytes and macrophages as measured by RNA-seq. Mono monocytes, Mac macrophages, RPM reads per million. n = 2. Source data are provided as a file. b Flow cytometry analysis of NRP2 versus IgG control cell surface staining in primary and THP1 monocytes and macrophages. c Flow cytometry analysis of THP1 macrophages with NRP2 or control CRISPR knockout, infected with HCMV-GFP. Analysis was performed at 3 dpi. d Flow cytometry analysis of THP1 macrophages, transfected with NRP2 and control siRNA 2 days before infection with HCMV-GFP. Analysis was performed at 3 dpi. Gating strategies for Fig. 6b–d are shown in Fig. .

    Article Snippet: FITC-conjugated mouse IgG1 anti-human CD29 (ITGB1) (Santa Cruz catalog no. MEM-101A) and FITC-conjugated mouse IgG1 control (Santa Cruz, catalog no. sc-2339).

    Techniques: Expressing, RNA Sequencing, Flow Cytometry, Control, Staining, CRISPR, Knock-Out, Infection, Transfection